He tikanga tātari kaha te Gas Chromatography-Mass Spectrometry (GC-MS) e whakakotahi ana i ngā āheinga wehewehe o te gas chromatography me te kaha tautuhi o te papatipu spectrometry. E whakamahia whānuitia ana tēnei tikanga mō te tātari kounga i roto i ngā momo mara, tae atu ki te aroturuki taiao, te haumaru kai, me te pūtaiao taihara. Ka matapakihia e tēnei tuhinga ngā mātāpono o te kimi pātengi raraunga papatipu spectrometry, ngā taupū pupuri, me ngā tikanga matatau i roto i te tātari GC-MS.
Wāhanga 1: Te Whakahoki i te Pūtake Raraunga o te Ine Papatipu
I tēnei wā, ko te nuinga o ngā pātengi raraunga ine papatipu Irahiko (EI) e hangaia ana mā te whakamahi i ngā ine papatipu whā-pū. Tērā pea ka whakaatuhia e ētahi atu momo ine papatipu ngā pānga wehewehe papatipu, ka arahi pea ki ngā hapa i roto i te tautuhi i ngā matū. I roto i te tātari GC-MS, he mea nui kia tangohia te haruru papamuri hei whakarei ake i te pono o ngā hua. Mā tēnei taahiraa ka whakarite kia rite ngā tohu i whiwhi ki ngā tātaritanga e hiahiatia ana, kaua ki ngā taonga tuku iho, ki ngā poke rānei.
I te wā e whiriwhiri ana i ngā irahiko papatipu hei tautuhi, me tūpato ki ngā take irahiko matū ka puta ake. Ka taea e ngā tikanga irahiko rerekē te whakaputa i ngā tauira wehewehenga rerekē, ka uaua ake te tukanga tautuhi. I roto i ngā tikanga irahiko papatipu rerekē, e mōhiotia ana te irahiko papatipu quadrupole mō tōna tika kimi teitei rawa atu, e kiia ana i te nuinga o te wā he 25%. Hei whakataurite, ko te irahiko papatipu mahanga irahiko e whai ake nei me te paku iti ake te tika, ko te irahiko papatipu Time-of-Flight (TOF) he iti ake ngā tere kimi. E whakaatu ana tēnei rerekētanga i te hiranga o te whiriwhiri i te tikanga irahiko papatipu tika i runga i ngā whakaritenga tātari motuhake.
Wāhanga 2: Ngā Mātāpono me ngā Tikanga o te Tātari Kounga o te Taupū Pupuri
Ko te taupū pupuri (RI) he tawhā nui i roto i te GC-MS e āwhina ana i te tautuhi kounga o ngā pūhui. Kei raro ko te GC o Diallyl trisulfide me 4-methylthiazole.
Taupū Pupuri i raro i ngā Āhuatanga Isothermal:
I roto i te whakatakotoranga chromatography hau pāmahana pumau, ka taea te whakaatu i te wā pupuri o ngā n-alkane e rua me ngā ngota waro n me N penei:
RI = 100n+100[Nn]*[log t'–log t'n]/[log t'N -log t'n]
t'= t-tm; t'n = tn-tm; ko te wā mate ko t'N = tN-tm.
Taupū Pupuri i raro i ngā Tikanga Whakamahere Pāmahana:
I roto i tētahi whakatakotoranga kua whakaritea mō te pāmahana, ka taea te tatau i te taupū pupuri mā te whakamahi i ngā wā pupuri tuturu hei utu mō ō rātou uara logarithmic: RI = 100n+100[Nn]*[t –tn]/[tN - tn]
Ngā Take e Pā Ana ki te Tāurangi Pupuri:
He maha ngā āhuatanga ka pā ki te taupū pupuri, tae atu ki te momo wāhanga tūmau e whakamahia ana, te kaupapa pāmahana, me te āhua o te matihiko tauira. He mea nui te mārama ki ēnei āhuatanga mō te tātari kounga tika.
Tikanga Kounga Pou Takirua:
Ko tēnei tikanga he tatau i ngā tohu pupuri o ngā wāhanga i runga i ngā āhua tūmau porowhita e rua. Mena ka ōrite ngā tohu pupuri e rua ki ngā tohu pupuri o ngā matū paerewa, ka taea te whakatutuki i te tautuhi kounga tuatahi.
Wāhanga 3: Ngā Tikanga Ine Papatipu Tandem
Mā te ine papatipu tandem (MS/MS) ka whakarei ake i te tātaritanga kounga mā te tuku i te wehewehenga o ngā katote, ka whakarato i ngā mōhiohio hanganga tāpiri. He tino whai hua tēnei tikanga mō ngā ranunga uaua, ka taea ai te tautuhi i ngā matū kāore pea e kitea i te wāhanga kotahi o te ine papatipu.
Wāhanga 4: Tātaritanga Kounga MS Taumira-Teitei
Mā te ine ine papatipu taumira teitei ka pai ake te tika me te taumira o te papatipu, ka waiho hei taputapu whai hua mō te tātari kounga. He tino whai hua tēnei tikanga ki te wehewehe i waenga i ngā isomers me ngā matū e pā tata ana, he mea nui tēnei i roto i te tātari tauira matatini.
Wāhanga 5: Tātaritanga Kounga GC-MS Tauhohenga Ion-Molecule
Mā ngā tauhohenga ira-ngota i roto i te GC-MS ka taea te whakarato māramatanga ki te tauhohenga me te hanganga o ngā pūhui pūngoi. He mea nui te whirihoranga katoa o ngā taputapu ine papatipu mō te tātaritanga wāhanga pūngoi mō ngā hua kounga tika. Ko ngā rautaki mō te tātaritanga kounga o ngā wāhanga pūngoi he maha ngā wā ka uru ki te whiriwhiri tupato i ngā tikanga ira me te arotau i ngā āhuatanga chromatography.
Hei whakamutunga, he tikanga matatau te GC-MS e whakakotahi ana i ngā āheinga wehewehe o te chromatography hau me te kaha tautuhi o te ine ira papatipu. Mā te whakamahi i te kimi pātengi raraunga ine ira papatipu, ngā tataunga pupuri taupū, me ngā tikanga matatau, ka taea e ngā kaitātari te whakatutuki i ngā hua kounga pono, ka waiho te GC-MS hei taputapu tino whai hua i roto i ngā momo kaupapa pūtaiao.
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